The antibody detects endogenous level of total eIF4G protein.
Anwendungen
WB, IHC, IF
Verdünnungen
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reaktivität
Human
Immunogen
Peptide sequence around aa.1230~1234 (P-V-S-P-L) derived from Human eIF4G.
Wirt
Rabbit
Klonalität
Polyclonal
Konjugat
Unconjugated
Reinigung
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Konzentration
1.0mg / ml
Geschätztes MW
220kd
Formulierung
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Lagerung
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (Ab-1232) Antibody #21514 (left) or the same antibody preincubated with blocking peptide (right).