Rabbit polyclonal antibody to eIF4EBP1 (phospho Ser65).
Anwendungen
WB, IP
Verdünnungen
WB: 1:500-1:1,000, IP: 1:50-1:100
Reaktivität
Human
Immunogen
A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65 (NP_004086.1).
Sequenz
RNSPV
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Affinity purification.
Molekulargewicht
20 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
4E-BP1, 4EBP1, 4EBP1_HUMAN, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Figure 1: Western Blot - Anti-eIF4EBP1 (phospho Ser65) Antibody (A16404)
Western blot analysis of extracts of 293T cells, using Anti-eIF4EBP1 (phospho Ser65) Antibody (A16404) at 1:1,000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37°C for 5 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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