Anti-eIF4E Antikörper (A309356)

Name: Anti-eIF4E Antibody
Brand: Antibodies.com
SKU: A309356
Price: 470.0 USD
Availability: InStock

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$470

Polyklonal Kaninchen Antikörper gegen eIF4E für WB, IHC und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-eIF4E Antibody

Beschreibung

Rabbit polyclonal antibody to eIF4E.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:100-1:500, ICC/IF: 1:50-1:200, IHC: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant protein of human eIF4E.

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

29 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

AUTS19, CBP, eIF 4E, EIF 4F, eIF 4F 25 kDa subunit, eIF-4E, eIF-4F 25 kDa subunit, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1, IF4E_HUMAN, Messanger RNA Cap Binding Protein eIF 4E, MGC111573, mRNA cap binding protein, mRNA cap-binding protein

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (9)

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Figure 1: Western Blot - Anti-eIF4E Antibody (A309356)

Western blot analysis of various lysates, using Anti-eIF4E Antibody (A309356) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-eIF4E Antibody (A309356)

Western blot analysis of extracts from wild type(WT) and eIF4E knockdown (KD) 293T(KD) cells, using Anti-eIF4E Antibody (A309356) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-eIF4E Antibody (A309356)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-eIF4E Antibody (A309356) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-eIF4E Antibody (A309356)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-eIF4E Antibody (A309356) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.