Anti-eIF4E Antikörper (A13922)

Name: Anti-eIF4E Antibody
Brand: Antibodies.com
SKU: A13922
Price: 470.0 USD
Availability: InStock

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$470

Polyklonal Kaninchen Antikörper gegen eIF4E für WB, IHC, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-eIF4E Antibody

Beschreibung

Rabbit polyclonal antibody to eIF4E.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4E (NP_001959.1).

Sequenz

MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEP

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

25 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

AUTS19, CBP, eIF 4E, EIF 4F, eIF 4F 25 kDa subunit, eIF-4E, eIF-4F 25 kDa subunit, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1, IF4E_HUMAN, Messanger RNA Cap Binding Protein eIF 4E, MGC111573, mRNA cap binding protein, mRNA cap-binding protein

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (6)

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Figure 1: Western Blot - Anti-eIF4E Antibody (A13922)

Western blot analysis of various lysates, using Anti-eIF4E Antibody (A13922) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

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Figure 2: Western Blot - Anti-eIF4E Antibody (A13922)

Western blot analysis of extracts from wild type(WT) and eIF4E knockout (KO) 293T(KO) cells, using Anti-eIF4E Antibody (A13922) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

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Figure 3: Immunohistochemistry - Anti-eIF4E Antibody (A13922)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-eIF4E Antibody (A13922) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-eIF4E Antibody (A13922)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-eIF4E Antibody (A13922) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.