Figure 1: Western Blot - Anti-eIF4B (phospho Ser422) Antibody (A91222)
Western blot analysis of extracts of HeLa cells, using Anti-eIF4B (phospho Ser422) Antibody (A91222) at 1:2,000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.
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