Anti-EGFR Antikörper (A81037)

Name: Anti-EGFR Antibody
Brand: Antibodies.com
SKU: A81037
Price: 335.0 USD
Availability: InStock

$335

Polyklonal Kaninchen Antikörper gegen EGFR für WB, IHC, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Stichworte:

Endosomal Marker ER Marker

Produktdetails

Produktname

Anti-EGFR Antibody

Beschreibung

Rabbit polyclonal antibody to EGFR.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:50-1:100, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).

Sequenz

QGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

175 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

9030024J15Rik, AI552599, Avian erythroblastic leukemia viral (v erb b) oncogene homolog , Avian erythroblastic leukemia viral (verbb) oncogene homolog , Cell growth inhibiting protein 40 , Cell proliferation inducing protein 61 , EGF R, EGFR_HUMAN, Epidermal growth factor receptor, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) , erb-b2 receptor tyrosine kinase 1, Erbb, erbb 1, Erbb1, Errp, Erythroblastic leukemia viral (v erb-b) oncogene homolog, HER1, mENA , NISBD2, Oncogen ERBB, Oncogene ERBB , OTTMUSP00000005385, OTTMUSP00000005386, PIG61, Proto oncogene c ErbB 1, Proto-oncogene c-ErbB-1, Receptor tyrosine protein kinase ErbB 1 , Receptor tyrosine protein kinase ErbB1 , Receptor tyrosine-protein kinase ErbB-1, RP23-295E4.1, SA7, Species antigen 7, Urogastrone, v-erb-b Avian erythroblastic leukemia viral oncogen homolog, wa2, Wa5

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (12)

Bild Vergrößern

Figure 1: Western Blot - Anti-EGFR Antibody (A81037)

Western blot analysis of extracts of A-549 cells, using Anti-EGFR Antibody (A81037) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-EGFR Antibody (A81037)

Western blot analysis of extracts of various cell lines, using Anti-EGFR Antibody (A81037) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Western Blot - Anti-EGFR Antibody (A81037)

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Figure 4: Immunohistochemistry - Anti-EGFR Antibody (A81037)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-EGFR Antibody (A81037) at a dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 5: Immunohistochemistry - Anti-EGFR Antibody (A81037)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-EGFR Antibody (A81037) at a dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 6: Immunohistochemistry - Anti-EGFR Antibody (A81037)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-EGFR Antibody (A81037) at a dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 7: Immunohistochemistry - Anti-EGFR Antibody (A81037)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord endothelial cells using Anti-EGFR Antibody (A81037) at a dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 8: Immunofluorescence - Anti-EGFR Antibody (A81037)

Immunofluorescence analysis of HeLa cells using Anti-EGFR Antibody (A81037) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 9: Immunofluorescence - Anti-EGFR Antibody (A81037)

Immunofluorescence analysis of NIH/3T3 cells using Anti-EGFR Antibody (A81037) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 10: Immunofluorescence - Anti-EGFR Antibody (A81037)

Immunofluorescence analysis of PC12 cells using Anti-EGFR Antibody (A81037) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 11: Western Blot - Anti-EGFR Antibody (A81037)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-EGFR Antibody (A81037). This Western blot was performed on the immunoprecipitate using Anti-EGFR Antibody (A81037) at a dilution of 1:1000.