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EdU Cell Proliferation Image Kit (Green Fluorescence) (A319642)

$205
Versandkosten
Vorlaufzeit
Lieferung in 7-8 Werktagen
Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com
Produktname
EdU Cell Proliferation Image Kit (Green Fluorescence)
Anwendungen
Fluorescence Microscopy
Assay-Prinzip
This kit is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, and is complete within 30 minutes.
Komponenten
EdU (10mM), BSA Wash Solution (5X), Fluor 488 azide, Reaction Buffer (10X), Copper Reagent, Reducing Agent, Hoechst 33342 (1000X), Hydroxyurea
Absorptionsmaxima
501 nm
Emissionsmaxima
525 nm
Lagerung
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
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