Mouse monoclonal [74.1] antibody to Dynein Cytoplasmic.
Specificity
This antibody recognises all known forms of the 74 kDa intermediate chain subunit of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the N-terminal portion of the molecule. Mouse anti Bovine dynein cytoplasmic 74 kDa antibody, clone 74.1 immunoprecipitates the intermediate chain subunits under denaturing conditions and the entire dynein complex under native conditions.
Applications
IHC-Fr, WB, IP
Dilutions
IHC-Fr: 1:10 - 1:100, WB: 1:1,000 - 1:5,000
Reactivity
Bovine, Fish, Mouse, Drosophila, Xenopus, Rat, Sheep
Cross Reactivity
This antibody does not cross react with Invertebrates
Immunogen
Native cytoplasmic dynein from bovine brain.
Host
Mouse
Clonality
Monoclonal
Clone ID
74.1
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of ascites.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Bovine dynein cytoplasmic 74 kDa antibody, clone 74.1> reacts with all known forms of the 74 kDa intermediate chain subunit of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the N-terminal portion of the molecule.Mouse anti Bovine dynein cytoplasmic 74 kDa antibody, clone 74.1 immunoprecipitates the intermediate chain subunits under denaturing conditions and the entire dynein complex under native conditions.
