Anti-Doublecortin Antikörper (A83146)

Name: Anti-Doublecortin Antibody
Brand: Antibodies.com
SKU: A83146
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Abcam (ab113435). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen Doublecortin für ELISA, WB, IHC und IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Stichworte:

Neuronal Marker

Produktdetails

Produktname

Anti-Doublecortin Antibody

Beschreibung

Goat polyclonal antibody to Doublecortin.

Anwendungen

ELISA, WB, IHC, IF

Verdünnungen

ELISA: 1:32,000, WB: 0.01-0.03µg/ml, IHC: 2-4µg/ml, IF: 5-10µg/ml

Reaktivität

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 232-242, of human Doublecortin.

Sequenz

C-KTSANMKAPQS

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

45 kDa

Geschätztes MW

49.3kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

DBCN, Dbct, DC, DCX, DCX_HUMAN, Doublecortex, Doublin, FLJ51296, Lis X, Lis-X, Lissencephalin X, Lissencephalin-X, Lissencephaly X linked, Lissencephaly X linked doublecortin, LISX, Neuronal migration protein doublecortin, OTTHUMP00000023859, OTTHUMP00000023860, OTTHUMP00000216315, OTTHUMP00000216316, SCLH, XLIS

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (6)

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Figure 1: Immunofluorescence - Anti-Doublecortin Antibody (A83146)

Doublecortin expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Doublecortin Antibody (A83146) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-Doublecortin Antibody (A83146)

Doublecortin expression in KNRK cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Doublecortin Antibody (A83146) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunohistochemistry - Anti-Doublecortin Antibody (A83146)

Doublecortin expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-Doublecortin Antibody (A83146) at 5µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 4: Immunohistochemistry - Anti-Doublecortin Antibody (A83146)

Negative control for Doublecortin expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.