Anti-Dnmt1 Antikörper (A88660)

Name: Anti-Dnmt1 Antibody
Brand: Antibodies.com
SKU: A88660
Price: 470.0 USD
Availability: InStock

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$470

Polyklonal Kaninchen Antikörper gegen Dnmt1 für WB, IHC, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

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Produktdetails

Produktname

Anti-Dnmt1 Antibody

Beschreibung

Rabbit polyclonal antibody to Dnmt1.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of DNMT1 (NP_001370.1).

Sequenz

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMAD

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

200 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

ADCADN, AIM, CXXC finger protein 9, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine 5 ) methyltransferase 1, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase 1, DNA methyltransferase HsaI, DNA methyltransferase M.HsaI., DNA MTase, DNA MTase HsaI, DNMT, DNMT 1, Dnmt1o, DNMT1_HUMAN, FLJ16293, HSN1E, M.HsaI, MCMT, Met1, MGC104992, mMmul, MommeD2

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

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Figure 1: Western Blot - Anti-Dnmt1 Antibody (A88660)

Western blot analysis of extracts of HeLa cells, using Anti-Dnmt1 Antibody (A88660) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-Dnmt1 Antibody (A88660)

Western blot analysis of extracts of various cell lines, using Anti-Dnmt1 Antibody (A88660) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-Dnmt1 Antibody (A88660)

Western blot analysis of extracts from wild type(WT) and DNMT1 knockdown (KD) 293T cells, using Anti-Dnmt1 Antibody (A88660) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 4: Immunohistochemistry - Anti-Dnmt1 Antibody (A88660)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Dnmt1 Antibody (A88660) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.