Synthetic peptide within amino acids 1-50 of human DDX6.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
200 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Buffered Saline with 0.1% BSA and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
ATP-dependent RNA helicase p54, DEAD box protein 6, HLR2, Oncogene RCK, Probable ATP-dependent RNA helicase DDX6, RCK
Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, MCF-7, Jurkat, and HepG2 cells prepared using NETN lysis buffer. Antibody: Anti-DDX6 Antibody (A295541) was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Anti-DDX6 Antibody (A295541) was used for IP at 6 µg per reaction. DDX6 was also immunoprecipitated using a previous batch of this antibody and a second antibody against a different epitope of DDX6. For blotting immunoprecipitated DDX6, Anti-DDX6 Antibody (A295541) was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Samples: Whole cell lysate (10 µg) from NIH-3T3, CT26, CH27, TCMK-1, and BW 5147.3 cells prepared using NETN lysis buffer. Antibody: Anti-DDX6 Antibody (A295541) was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
![Western Blot - Anti-DDX6 Antibody [ARC1673] (A309192) - Antibodies.com](https://cdn.antibodies.com/image/catalog/309/A309192_1.jpg?profile=product_alternative)
