Figure 1: Western Blot - Anti-DDDDK Tag Antibody (A17320)
Western blot analysis of 293T-GFP-His-Myc-Flag-HA protein using Anti-DDDDK Tag Antibody (A17320) at different dilutions. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST.
Figure 2: Immunofluorescence - Anti-DDDDK Tag Antibody (A17320)
Immunofluorescence analysis of 293T cells transfected with Flag-c(mCherry) and 293T cells transfected with Flag-N(mCherry) using Anti-DDDDK Tag Antibody (A17320) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 3: Immunofluorescence - Anti-DDDDK Tag Antibody (A17320)
Immunofluorescence analysis of 293T cells transfected with Flag-N(mCherry) and untreated 293T cells using Anti-DDDDK Tag Antibody (A17320) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 4: Western Blot - Anti-DDDDK Tag Antibody (A17320)
Immunoprecipitation analysis of 200µg extract cell lysate from 293T cells transfected with GSK3B expression vector containing a DDDDK-Tag using 3µg of Anti-DDDDK Tag Antibody (A17320). This Western blot was performed on the immunoprecipitate using Anti-DDDDK Tag Antibody (A17320)at 1:1,000 dilution.
Figure 5: Western Blot - Anti-DDDDK Tag Antibody (A17320)
Immunoprecipitation analysis of 300µg extracts of 293T-SERPINB1-Flag-GFP cells using 3µg of Anti-DDDDK Tag Antibody (A17320). This Western blot was performed on the immunoprecipitate using Anti-DDDDK Tag Antibody (A17320) at a dilition of 1:50000.
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