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Anti-Cytokeratin 8 Antikörper (A12853)

Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.com
$335
Product Datasheet

Polyklonal Kaninchen Antikörper gegen Cytokeratin 8 für WB, IHC und ICC/IF.

100% Qualitätsgarantie
Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 5-8 Werktagen

Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
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orders@antibodies.com

Produktname
Anti-Cytokeratin 8 Antibody
Beschreibung
Rabbit polyclonal antibody to Cytokeratin 8.
Anwendungen
WB, IHC, ICC/IF
Verdünnungen
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reaktivität
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-483 of human Cytokeratin 8 (KRT8) (NP_002264.1).
Sequenz
MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
54 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
CARD2, CK 8, CK-8, CK8, CYK8, CYKER, Cytokeratin endo A, Cytokeratin-8, DreK8, EndoA, K2C8, K2C8_HUMAN, K8, Keratin 8, Keratin type II cytoskeletal 8, Keratin, type II cytoskeletal 8, Keratin-8, KO, Krt 2.8, KRT8, MGC118110, MGC174782, MGC53564, MGC85764, sb:cb186, Type-II keratin Kb8
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-Cytokeratin 8 Antibody (A12853)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 8 Antibody (A12853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comBild Vergrößern
Figure 2: Western Blot - Anti-Cytokeratin 8 Antibody (A12853)
Western blot analysis of extracts of Rat liver, using Anti-Cytokeratin 8 Antibody (A12853) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comBild Vergrößern
Figure 3: Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 8 Antibody (A12853) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853) - Antibodies.comBild Vergrößern
Figure 4: Immunohistochemistry - Anti-Cytokeratin 8 Antibody (A12853)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Cytokeratin 8 Antibody (A12853) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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