Anti-Cytokeratin 5 Antikörper (A14122)

Name: Anti-Cytokeratin 5 Antibody
Brand: Antibodies.com
SKU: A14122
Price: 335.0 USD
Availability: InStock

Bild Vergrößern

$335

Polyklonal Kaninchen Antikörper gegen Cytokeratin 5 für WB, IHC, ICC/IF und IP.

100% Qualitätsgarantie

Preis-Match-Garantie

Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Produktdetails

Produktname

Anti-Cytokeratin 5 Antibody

Beschreibung

Rabbit polyclonal antibody to Cytokeratin 5.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:2,000-1:6,000

Reaktivität

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 5 (KRT5) (NP_000415.2).

Sequenz

MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGG

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

62 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

58 kDa cytokeratin, CK-5, CK5, Cytokeratin-5, Cytokeratin5, DDD, DDD1, EBS2, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K2C5_HUMAN, K5, Keratin, Keratin 5, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), keratin complex 2, basic, gene 5, keratin, type II cytoskeletal 5, Keratin-5, Keratin5, KRT 5, Krt5, KRT5A, type II cytoskeletal 5, Type-II keratin Kb5

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

Bild Vergrößern

Figure 1: Western Blot - Anti-Cytokeratin 5 Antibody (A14122)

Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 5 Antibody (A14122) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Bild Vergrößern

Figure 2: Immunohistochemistry - Anti-Cytokeratin 5 Antibody (A14122)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Bild Vergrößern

Figure 3: Immunofluorescence - Anti-Cytokeratin 5 Antibody (A14122)

Immunofluorescence analysis of L929 cells using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

Bild Vergrößern

Figure 4: Western Blot - Anti-Cytokeratin 5 Antibody (A14122)

Immunoprecipitation analysis of 200µg extracts of A-431 cells using 3µg of Anti-Cytokeratin 5 Antibody (A14122). This Western blot was performed on the immunoprecipitate using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:5000.