Rabbit monoclonal [ARC1559] antibody to Cyclin E1 (phospho Thr77).
Anwendungen
WB
Verdünnungen
WB: 1:500-1:2,000
Reaktivität
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around T77 of human Cyclin E1 (P24864).
Sequenz
IPTPD
Wirt
Rabbit
Klonalität
Monoclonal
Klon
ARC1559
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Affinity purification.
Molekulargewicht
54 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Figure 1: Western Blot - Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326) at 1:1,000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.
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