Rabbit polyclonal antibody to Cyclin E1 (phospho Thr77).
Spezifität
This antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Thr77.
Anwendungen
IF, ELISA
Verdünnungen
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:40000
Reaktivität
Human
Immunogen
Synthetic peptide derived from human Cyclin E1 around the phosphorylation site of Thr77 (amino acids 43-92).
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.
Molekulargewicht
47kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Immunofluorescence analysis of HeLa cells using Anti-Cyclin E1 (phospho Thr77) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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