Anti-Cyclin D1 Antikörper [ARC0300] (A306339)

Name: Anti-Cyclin D1 Antibody [ARC0300]
Brand: Antibodies.com
SKU: A306339
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC0300] Kaninchen Antikörper gegen Cyclin D1 für WB, IHC, ICC/IF und IP.

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Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

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+1 (314) 370-6046

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Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-Cyclin D1 Antibody [ARC0300]

Beschreibung

Rabbit monoclonal [ARC0300] antibody to Cyclin D1.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).

Sequenz

ISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC0300

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

34 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

AI327039, B cell CLL/lymphoma 1, B cell leukemia 1, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL 1, BCL-1, BCL-1 oncogene, BCL1, BCL1 oncogene, ccnd1, CCND1/FSTL3 fusion gene, included, CCND1/IGHG1 fusion gene, included, CCND1/IGLC1 fusion gene, included, CCND1/PTH fusion gene, included, CCND1_HUMAN, cD1, Cyl 1, D11S287E, G1/S specific cyclin D1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1, PRAD1 oncogene, U21B31

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (10)

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Figure 1: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Western blot analysis of extracts from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 2: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Western blot analysis of extracts of various cell lines, using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 3: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 5: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 6: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 7: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 8: Immunofluorescence - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunofluorescence analysis of C6 cells using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 9: Immunofluorescence - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 10: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-Cyclin D1 Antibody [ARC0300] (A306339). This Western blot was performed on the immunoprecipitate using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:1000.

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Monoklonal [ARC0300] Kaninchen Antikörper gegen Cyclin D1 für WB, IHC, ICC/IF und IP.

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