Figure 1: Western Blot - Anti-CROT/COT Antibody (A84938)
CROT/COT expression in Rat Kidney lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CROT/COT Antibody (A84938) at 0.3µg/ml and detected by chemiluminescence.
CROT/COT expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-CROT/COT Antibody (A84938) at 5µg/ml and revealed with alkaline phosphatase (AP).
CROT/COT expression in Human Liver analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-CROT/COT Antibody (A84938) at 5µg/ml and revealed with alkaline phosphatase (AP).