Figure 1: Western Blot - Anti-CRB1 Antibody (A8756)
Western blot analysis of extracts of HeLa cells, using Anti-CRB1 Antibody (A8756) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded mouse retina using Anti-CRB1 Antibody (A8756) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
