Figure 1: Immunofluorescence - Anti-Collagen IV alpha 6 Antibody (A286021)
Collagen IV alpha 6 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Collagen IV alpha 6 Antibody (A286021) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Figure 2: Immunofluorescence - Anti-Collagen IV alpha 6 Antibody (A286021)
Collagen IV alpha 6 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Collagen IV alpha 6 Antibody (A286021) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Collagen IV alpha 6 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Collagen IV alpha 6 Antibody (A286021) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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