Immunofluorescent analysis of rat cerebellum section stained with Anti-CNPase Antibody (1:500 | green) and Anti-NF-M Antibody (A85324 | 1:1,000 | red). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies. The Anti-CNPase Antibody stains the oligodendrocytes, cells that create the myelin sheath around axons. Anti-NF-M Antibody labels axons of neuronal cells.
Mixed neuron-glial cell cultures stained with Anti-CNPase Antibody (red) and Anti-GFAP Antibody (A85419 | green). The Anti-CNPase Antibody stains strongly in oligodendrocytes, whereas Anti-GFAP Antibody labels only the intermediate filaments in astrocytes. Blue is DNA staining.
Figure 3: Western Blot - Anti-CNPase Antibody [1H10] (A85413)
Western blot analysis of different tissue lysates using Anti-CNPase Antibody (1:2,000 | green): [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Double bands at 46, 48 kDa mark correspond to isotypes of the CNP protein.
Figure 4: Western Blot - Anti-CNPase Antibody [1H10] (A85413)
Blots of rat brain tissue homogenates probed with Anti-CNPase Antibody at 1:5,000 (Lane 1) and 1:20,000 (Lane 2). The antibody binds strongly and cleanly to a band at ~48kDa.
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