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Anti-Clostridium Difficile Toxin B Aptamer (Biotin) (A321083)

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Vorlaufzeit
Lieferung in 30-45 Werktagen
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+1 (314) 370-6046
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Email
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Name
Anti-Clostridium Difficile Toxin B Aptamer (Biotin)
Description
Synthetic DNA aptamer to Clostridium Difficile Toxin B (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 90 °C for 15 min and then cooled on ice for 15 min.
Apatmer Binding Conditions
20mM Tris-HCl Buffer, pH 7.5, with 100mM Sodium Chloride, 5mM Potassium Chloride and 10mM Magnesium Chloride, 1mM DTT, and 0.05% Tween 20. Binding Temperature: 25°C.
Sequence
5'-dCpdTpdCpdCpdCpdApdTpdGpdCpdTpdApdTpdTpdApdTpdGpdGpdCpdTpdApdGpdCpdGpdCpdApdGpdGpdApdGpdGpdCpdCpdApdCpdApdCpdCpdGpdGpdAp-3'
Aptamer Length
120 nt
Aptamer Molecular Weight (Da)
12,266.98 Da
Aptamer Affinity
Affinity (Kd) = 8.9 nM
Extinction Coefficient
419360 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Clostridium Difficile Toxin B Aptamer (Biotin) (A321083) is a replica of the aptamer described in PMID: 32155319.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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