Western Blot - Anti-Chk2 Antibody [ARC57076] (A306128)
Western blot analysis of HeLa, using Anti-Chk2 Antibody [ARC57076] (A306128) at 1:7,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-Chk2 Antibody [ARC57076] (A306128)
Western blot analysis of C2C12, using Anti-Chk2 Antibody [ARC57076] (A306128) at 1:7,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Chk2 Antibody [ARC57076] (A306128) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human testis using Anti-Chk2 Antibody [ARC57076] (A306128) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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