Rabbit polyclonal antibody to Chk1 (Phospho-Ser296)
Specificity
The antibody detects endogenous levels of Chk1 only when phosphorylated at serine 296.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 296(I-Q-S(p)-N-L) derived from Human Chk1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HUVEC cells treated with UV using Chk1 (Phospho-Ser296) Antibody #11673.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody #11673 (left)or the same antibody preincubated with blocking peptide (right).
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