Anti-Chk1 (phospho Ser286) Antibody (A95277)

Name: Anti-Chk1 (phospho Ser286) Antibody
Brand: Antibodies.com
SKU: A95277
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to Chk1 (phospho Ser286) for WB, IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-Chk1 (phospho Ser286) Antibody

Description

Rabbit polyclonal antibody to Chk1 (phospho Ser286).

Specificity

This antibody detects endogenous levels of Chk1 only when phosphorylated at Ser286.

Applications

WB, IF, ELISA

Dilutions

WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:5000

Reactivity

Human

Immunogen

Synthetic peptide derived from human Chk1 around the phosphorylation site of Ser286 (amino acids 256-305).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

54kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Immunofluorescence - Anti-Chk1 (phospho Ser286) Antibody (A95277)

Immunofluorescence analysis of HeLa cells using Anti-Chk1 (phospho Ser286) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-Chk1 (phospho Ser286) Antibody (A95277)

Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40' using Anti-Chk1 (phospho Ser286) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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