Anti-Chk1 (phospho Ser280) Antibody (A12451)

Name: Anti-Chk1 (phospho Ser280) Antibody
Brand: Antibodies.com
SKU: A12451
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to Chk1 (phospho Ser280) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Chk1 (phospho Ser280) Antibody

Description

Rabbit polyclonal antibody to Chk1 (phospho Ser280).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S280 of human Chk1 (NP_001265.2).

Sequence

VTSGG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Chk1 (phospho Ser280) Antibody (A12451)

Western blot analysis of 293T, using Anti-Chk1 (phospho Ser280) Antibody (A12451) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-Chk1 (phospho Ser280) Antibody (A12451)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Chk1 (phospho Ser280) Antibody (A12451) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Chk1 (phospho Ser280) Antibody (A12451)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Chk1 (phospho Ser280) Antibody (A12451) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Chk1 (phospho Ser280) Antibody (A12451)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Chk1 (phospho Ser280) Antibody (A12451) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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