This ELISA kit is indirect enzyme-linked immune-sorbent assay technology. The recombinant HPV16 L1 antigens were pre-coated onto 96-well plates. The test samples were added to the wells and incubated subsequently, the HPV16 L1 antibodies in the test samples combined to the pre-coated antigens specifically, then followed by washing with wash buffer to remove unspecific components. Next, the HRP conjugated antibodies were added and incubated to bind to chicken antibodies in plate, the unbound conjugates were washed away with wash buffer. TMB substrate were used to visualize HRP enzymatic reaction. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Serum, egg white, and chicken derived samples.
Reaktivität
Chicken
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at 2-8°C for 6 months or at -20°C for 12 months Store secondary antibody at 2-8°C.
Synonyms
HPV-16, HPV-16 capsid, HPV16 capsid protein, HPV16 major capsid protein L1, Human papillomavirus type 16 L1, Human papillomavirus type 16 major capsid protein L1, L1, Major capsid L1 protein, Major capsid protein, Major capsid protein L1
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
