This antibody reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

Anwendungen

Flow Cytometry, IP, WB, IHC-P, ICC

Verdünnungen

Flow Cytometry: 1-4 µg/ml.

Reaktivität

Human

Immunogen

C91/PL (human HTLV-1+ T cell line).

Wirt

Mouse

Klonalität

Monoclonal

Klon

C33

Isotyp

IgG2a

Konjugat

Unconjugated

Reinigung

Protein A chromatography.

Konzentration

1 mg/ml

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

4F9, Antigen detected by monoclonal and, C33, C33 antigen, CD 82, CD82 antigen, CD82 molecule, CD82_HUMAN, GR 15, GR15, IA 4, IA4, Inducible membrane protein, Inducible membrane protein R2, KAI 1, KAI1, Kangai 1, Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and, Kangai1, Leukocyte surface antigen R2, Metastasis suppressor Kangai 1, Metastasis suppressor Kangai-1, Metastasis suppressor Kangai1, Prostate cancer antimetastasis gene KAI1, R2, R2 leukocyte antigen, SAR 2, SAR2, ST 6, ST6, Suppression of tumorigenicity 6, Suppression of tumorigenicity 6 prostate, Suppressor of tumorigenicity 6, Suppressor of tumorigenicity 6 protein, Tetraspanin 27, Tetraspanin-27, Tetraspanin27, Tspan 27, Tspan-27, Tspan27

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (1)

Bild Vergrößern

Figure 1: Immunohistochemistry - Anti-CD82 Antibody [C33] (A86572)

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with Anti-CD82 Antibody (A86572).

Produktreferenzen für diesen Klon (1)

Bild Vergrößern

(6)

Overexpression of KAI1/CD82 suppresses in vitro cell growth, migration, invasion and xenograft growth in oral cancer.

Verweise: Anti-CD82 Antibody [C33] (ab140238)

Abstrakt:

KAI1/CD82 is a metastatic suppressor gene in human prostate cancer and several other types of cancer in humans. The present study aimed to examine the role of the overexpression of KAI1 in the progression of oral cancer. Human KAI1/CD82 cDNA was transfected into OSCC‑15 and 293T cell lines, and its effects on OSCC‑15 cell proliferation, invasion and apoptosis were assessed by performing a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, Matrigel invasion and Annexin V‑FITC staining, respectively. In addition, a xenograft model was used to assess the effect of KAI1/CD82 on the in vivo growth of tumors. The overexpression of KAI1/CD82 inhibited the proliferation and invasion of OSCC-15 cells. It also enhanced the apoptotic rate of the OSCC‑15 cells. Furthermore, the overexpression of KAI1/CD82 inhibited tumor growth in the xenograft model. The results demonstrated that the overexpression of KAI1/CD82 significantly inhibited the proliferation and invasion of human oral cancer cells, and inhibited tumor growth in the xenograft model. Therefore, KAI1/CD82 may be considered as a potential therapeutic target in oral cancer.

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