This antibody recognises guinea pig CD8 present on cytotoxic T-cells. CD8 comprises 2 subunits, alpha and beta and exists as either an alpha/alpha homodimer or an alpha/beta heterodimer. Sequence analysis suggests that guinea pig CD8 is more closely related to human than rat or mouse CD8.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Guinea Pig
Immunogen
Guinea Pig peritoneal T-cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
CT6
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Guinea Pig CD8 antibody, clone CT6 reacts with guinea pig CD8 present on cytotoxic T-cells. CD8 comprises 2 subunits, alpha and beta and exists as either an alpha/alpha homodimer or an alpha/beta heterodimer. Sequence analysis suggests that guinea pig CD8 is more closely related to human than rat or mouse CD8 (Nagarajan et al. 2004).