This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Anwendungen
IHC-P
Verdünnungen
IHC-P: 1-2 µg/ml
Reaktivität
Human, Monkey, Mouse, Rat, Rabbit, Feline
Kreuzreaktivität
This antibody does not cross react with Porcine, Canine, or Chicken.
Immunogen
Subcellular fraction of human alveolar macrophages.
Wirt
Mouse
Klonalität
Monoclonal
Klon
KP1
Isotyp
IgG1
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
1 mg/ml
Molekulargewicht
110 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-CD68 Antibody [KP1] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
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