This antibody recognises human CD68 an integral membrane glycoprotein of ~110 kDa also known as Macrosialin or Gp110. CD68 is predominantly expressed on the intracellular lysosomes of macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including Acute Myeloid Leukemia. In immunohistochemistry, CD68 can be used to aid in the identification of blastic NK lymphomas, some B cell lymphomas, and to help diagnose disorders relating to macrophage abnormalities, including malignant histocytosis and Gaucher’s disease. Clone Ki-M7 has also been reported as being suitable for use in immunoprecipitation.
Anwendungen
Flow Cytometry
Verdünnungen
Use 20µl of the suggested working dilution to label 106 cells in 100µl
Reaktivität
Human, African green monkey
Immunogen
Human lymph node tissue.
Wirt
Mouse
Klonalität
Monoclonal
Klon
Ki-M7
Isotyp
IgG1
Konjugat
FITC
Excitation: 490nm, Emission: 525nm
Reinigung
Protein A affinity chromatography of tissue culture supernatant.
Konzentration
Lot Specific
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 0.05% Sodium Azide and 1% BSA.
Lagerung
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Allgemeine Hinweise
Mouse anti Human CD68 antibody, clone Ki-M7 recognizes human CD68 an integral membrane glycoprotein of ~110 kDa also known as Macrosialin or Gp110. CD68 is predominantly expressed on the intracellular lysosomes of macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including Acute Myeloid Leukemia. In immunohistochemistry, CD68 can be used to aid in the identification of blastic NK lymphomas, some B cell lymphomas, and to help diagnose disorders relating to macrophage abnormalities, including malignant histocytosis and Gaucher’s disease. Clone Ki-M7 has also been reported as being suitable for use in immunoprecipitation.
Synonyme
CD 68, CD68 antigen, CD68 molecule, CD68_HUMAN, DKFZp686M18236, gp11, Gp110, LAMP4, MACROPHAGE ANTIGEN CD68, Macrophage antigen CD68 (microsialin), Macrosialin, SCARD1, Scavenger receptor class D member 1
