Anti-CD68 Antikörper [ED1] (PE) (A280319)

Anti-CD68 Antibody [ED1] (PE)

Mouse monoclonal [ED1] antibody to CD68 (PE).

This antibody recognises the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68. The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat. ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface. The expression of ED1 antigen being predominantly cytoplasmic , flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.

Flow Cytometry

FC 1: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Rat, Bovine

This antibody does not cross react with Horse

Rat spleen cells.

Mouse

Monoclonal

ED1

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti rat CD68 antibody, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68 (Dijkstra et al. 1985).The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.

CD 68, CD68 antigen, CD68 molecule, CD68_HUMAN, DKFZp686M18236, gp11, Gp110, LAMP4, MACROPHAGE ANTIGEN CD68, Macrophage antigen CD68 (microsialin), Macrosialin, SCARD1, Scavenger receptor class D member 1

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.