Mouse monoclonal [4H11[APG]] antibody to CD34 (PE-DyLight® 594).
Spezifität
This antibody reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells. It is gradually lost on progenitor cells. This antibody completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Anwendungen
Flow Cytometry
Verdünnungen
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reaktivität
Human
Immunogen
Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Wirt
Mouse
Klonalität
Monoclonal
Klon
4H11[APG
Isotyp
IgG1
Konjugat
PE-DyLight® 594
Reinigung
This antibody is conjugated with tandem dye PE-DyLight® 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Geschätztes MW
110-115 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Lagerung
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!