This antibody recognises mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow.

Anwendungen

Flow Cytometry

Verdünnungen

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Reaktivität

Mouse

Immunogen

BALB/c macrophage precursor cell hybrids.

Wirt

Rat

Klonalität

Monoclonal

Klon

ER-MP12

Isotyp

IgG2a

Konjugat

Biotin

Reinigung

Protein G affinity chromatography of tissue culture supernatant.

Konzentration

100 µg/ml

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Lagerung

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Allgemeine Hinweise

Rat anti Mouse CD31 antibody, clone ER-MP12 recognizes mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).

Synonyme

Adhesion molecule, CD31 antigen, CD31 EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', GPIIA'', HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM, PECAM 1, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule 1

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.