Recombinant rabbit monoclonal [Ki-1/1747R] antibody to CD30.
Spezifität
This antibody recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkins disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
Anwendungen
IHC-P
Verdünnungen
IHC-P: 1-2 µg/ml
Reaktivität
Human
Immunogen
Recombinant full-length human CD30 protein.
Wirt
Rabbit
Klonalität
Monoclonal
Klon
Ki-1/1747R
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Protein A chromatography.
Konzentration
200 µg/ml
Molekulargewicht
105-120 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-CD30 Antibody [Ki-1/1747R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
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