Recombinant rabbit monoclonal [DM104] antibody to CD30.
Anwendungen
ELISA, Flow Cytometry
Verdünnungen
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reaktivität
Human
Wirt
Rabbit
Klonalität
Monoclonal
Klon
DM104
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Affinity chromatography.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with distilled sterile water.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Allgemeine Hinweise
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyme
CD 30, CD30 antigen, CD30L receptor, Cytokine receptor CD30, D1S166E, KI 1, KI 1 antigen, Ki-1 antigen, KI1, Lymphocyte activation antigen CD30, TNFRSF 8, Tnfrsf8, TNR8_HUMAN, Tumor necrosis factor receptor superfamily member 8
ELISA plates were pre-coated with Recombinant Human CD30 Protein (6×His Tag) (A318247) at 2 µg/ml (100 µl/well) which can bind Anti-CD30 Antibody [DM104] - BSA and Azide free (A317355) in a linear range of 0.12-80 ng/ml.
Flow cytometry analysis of Anti-CD30 Antibody [DM104] - BSA and Azide free (A317355) on Expi293 cells transfected with human CD30 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of Anti-CD30 Antibody [DM104] - BSA and Azide free (A317355). (A) Anti-CD30 Antibody [DM104] - BSA and Azide free (A317355) does not bind to 293T cells that do not express CD30. (B) A clear peak shift of Anti-CD30 Antibody [DM104] - BSA and Azide free (A317355) was seen compared to the control when incubated with CD30-expressing 8226 cells, indicating strong binding of Anti-CD30 Antibody [DM104] - BSA and Azide free (A317355) to CD30. Antibodies were incubated at 10 µg/ml. .
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