Anti-CD3 zeta (phospho Y142) Antikörper [EM-54] (A86525)

Name: Anti-CD3 zeta (phospho Y142) Antibody [EM-54]
Brand: Antibodies.com
SKU: A86525
Price: 195.0 USD
Availability: InStock

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$195

Monoklonal [EM-54] Maus Antikörper gegen CD3 zeta (phospho Y142) für Flow Cytometry und WB.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 4-6 Werktagen

Kontakt

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+1 (314) 370-6046

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Produktdetails

Produktname

Anti-CD3 zeta (phospho Y142) Antibody [EM-54]

Beschreibung

Mouse monoclonal [EM-54] antibody to CD3 zeta (phospho Y142).

Spezifität

This antibody recognizes phosphorylated intracellular tyrosine 142 of CD3 zeta chain, which is a component of TCR/CD3 complex expressed on T cells.

Anwendungen

Flow Cytometry, WB

Verdünnungen

Flow Cytometry: 1-4 µg/ml; Intracellular staining.

Reaktivität

Human, Mouse

Immunogen

CP1 (pTyr142) - KLH.

Wirt

Mouse

Klonalität

Monoclonal

Klon

EM-54

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein A chromatography.

Konzentration

1 mg/ml

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

4930549J05Rik, A430104F18Rik, AW552088, CD247, CD247 antigen, Cd247 molecule, Cd3, CD3 antigen, zeta polypeptide, isoform CRA_b, CD3 antigen, zeta subunit, CD3-eta, CD3H, CD3Q, Cd3z, CD3Z antigen zeta polypeptide (TiT3 complex), CD3zeta, CD3zeta chain, CD3Z_HUMAN, MGC140430, T cell receptor T3 zeta chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3z, TCR zeta chain, TCRk, TCRZ, TCRzeta

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (1)

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Figure 1: Western Blot - Anti-CD3 zeta (phospho Y142) Antibody [EM-54] (A86525)

Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with Anti-CD3 zeta (Phospho-Tyr142) Antibody (A86525). 1: Wt mCD3zeta pervanadate non-stimulated. 2: Wt mCD3zeta pervanadate stimulated. 3: Mut. mCD3zeta Y/F2-6. 4: Mut. mCD3zeta Y/F1 and 3-6. 5: Mut. mCD3zeta Y/F1-2 and 4-6. 6: Mut. mCD3zeta Y/F1-3 and 5-6. 7: Mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native). 8: Mut. mCD3zeta Y/F1-5.