Anti-CD3 zeta (phospho Y142) Antibody [EM-54] (A86525)

Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with Anti-CD3 zeta (Phospho-Tyr142) Antibody (A86525). 1: Wt mCD3zeta pervanadate non-stimulated. 2: Wt mCD3zeta pervanadate stimulated. 3: Mut. mCD3zeta Y/F2-6. 4: Mut. mCD3zeta Y/F1 and 3-6. 5: Mut. mCD3zeta Y/F1-2 and 4-6. 6: Mut. mCD3zeta Y/F1-3 and 5-6. 7: Mut. mCD3zeta Y/F1-4 and 6 (thus only pY142 remains native). 8: Mut. mCD3zeta Y/F1-5.