Anti-CD3 zeta Antibody [ARC0533] (A308361)

Western blot analysis of extracts of Jurkat cells, using Anti-CD3 zeta Antibody [ARC0533] (A308361) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CD3 zeta Antibody [ARC0533] (A308361) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Flow cytometry analysis of Jurkat cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD3 zeta Antibody [ARC0533] (A308361), (10 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line).

Flow cytometry analysis of Raji cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD3 zeta Antibody [ARC0533] (A308361), (2.5 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Raji cells was used as blank control (red line).