Anti-CD3 epsilon Antibody [CD3-12] (A280087)

Anti-CD3 epsilon Antibody [CD3-12]

Rat monoclonal [CD3-12] antibody to CD3 epsilon.

This antibody recognises human CD3e. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (e, ?, d, ?) that assemble and function as three pairs of dimers (e?, ed, ??). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).

WB, IHC-P, IF, Flow Cytometry, IHC-Fr

FC 1: 1:50 - 1:100, IHC-Fr: 1:100, IHC-P 2: 1:100

Human, Bovine, Dog, Horse, Rhesus Monkey, Pig, Chicken, Mouse, Duck, Koala, Harbour Porpoise, Alpaca, Cynomolgus monkey, Spotted Hyena, Sea Lion, Cat, Amazon Parrot, Mammals, Birds, Raccoon, Great horned owl (Bubo virginianus), Bullfrog, Xenopus, Amphibia, Rabbit, African green monkey

Synthetic peptide sequence ERPPPVPNPDYEPC from cytoplasmic epitope of CD3.

ERPPPVPNPDYEPC

Rat

Monoclonal

CD3-12

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Human CD3 antibody, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3e chain recognizes human CD3e. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (e, ?, d, ?) that assemble and function as three pairs of dimers (e?, ed, ??). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).

CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E