This antibody recognises mouse CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Anwendungen
Flow Cytometry
Verdünnungen
Flow Cytometry: Neat - 1:10, Use10ul of the suggested working dilution to label 106 cells in 100µl.
Reaktivität
Mouse
Immunogen
Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Wirt
Mouse
Klonalität
Monoclonal
Klon
E18
Isotyp
IgG2b
Konjugat
PE
Excitation: 565nm, Emission: 578nm
Reinigung
Protein G affinity chromatography of tissue culture supernatant.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with 1 ml of distilled water
Formulierung
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Lagerung
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Allgemeine Hinweise
Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Synonyme
CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44