Anti-CD28 Antikörper [CC220] (A280022)

Anti-CD28 Antibody [CC220]

Mouse monoclonal [CC220] antibody to CD28.

This antibody recognises the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Mouse anti Bovine CD28, clone CC220 is able to stimulate bovine T lymphocytes in combination with anti-bovine CD3. For functional use, the use of the preservative free format (MCA5778EL) is recommended. Mouse anti Bovine CD28, clone CC220 has also been published under the alternative designation F849CD10 (Hogg et al. 2011 & Guzman et al. 2014).

Flow Cytometry, Functional Assays

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Bovine

Plasmid DNA encoding the bovine CD28 gene.

Mouse

Monoclonal

CC220

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Bovine CD28, clone CC220 recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). Mouse anti Bovine CD28, clone CC220 is able to stimulate bovine T lymphocytes in combination with anti-bovine CD3 (Nishimori et al. 2017).For functional use, the use of the preservative free format (MCA5778EL) is recommended. Mouse anti Bovine CD28, clone CC220 has also been published under the alternative designation F849CD10 (Hogg et al. 2011 & Guzman et al. 2014).

CD 28, CD28 antigen, CD28 molecule, CD28_HUMAN, MGC138290, T cell antigen CD28, T cell specific surface glycoprotein, T cell specific surface glycoprotein CD28, T-cell-specific surface glycoprotein CD28, TP44