Anti-CD276 Antibody [DM53] (Biotin) - BSA and Azide free
Beschreibung
Recombinant rabbit monoclonal [DM53] antibody to CD276 (Biotin).
Anwendungen
ELISA, Flow Cytometry
Verdünnungen
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reaktivität
Human
Wirt
Rabbit
Klonalität
Monoclonal
Klon
DM53
Isotyp
IgG
Konjugat
Biotin
Reinigung
Affinity chromatography.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with distilled sterile water.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Allgemeine Hinweise
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
1e5 of Expi 293 cell line were stained with 100µl of 1:1,000 diluted Anti-CD276 Antibody [DM53] (Biotin) - Azide free (A318642) (blue histogram) or isotype control (red histogram) respectively, washed and then stained with Streptavidin APC. The experimental samples were analyzed by flow cytometry.