This antibody recognises domain 2 of the murine cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the B-cell antigen receptor.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Reactivity
Mouse
Host
Rat
Clonality
Monoclonal
Clone ID
OX-97
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Rat anti Mouse CD22 antibody, clone OX-97 recognizes domain 2 of the murine cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the B-cell antigen receptor.
