This antibody reacts with an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells. It is also expressed by all thymocytes.

Anwendungen

Flow Cytometry

Verdünnungen

Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reaktivität

Human

Immunogen

Normal human blood lymphocytes.

Wirt

Mouse

Klonalität

Monoclonal

Klon

LT2

Isotyp

IgG2b

Konjugat

Excitation: 565nm, Emission: 578nm

Reinigung

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Geschätztes MW

50 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Lagerung

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyme

CD 2, CD2 antigen, CD2 antigen (p50), sheep red blood cell receptor, CD2 molecule, CD2_HUMAN, Erythrocyte receptor, FLJ46032, LFA-2, LFA-3 receptor, LFA2, LFA3 receptor, Ly-37, Lymphocyte function antigen 2, lymphocyte-function antigen-2, OTTHUMP00000024366, Rosette receptor, Sheep erythrocyte receptor, SRBC, T cell surface antigen CD2, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5, T-lymphocyte surface CD2 antigen, T11

Isotyp-Kontrollantikörper

Mouse IgG2b [MPC-11] (PE) (A86382)

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktreferenzen für diesen Klon (1)

Publikation anzeigen

[A panel of monoclonal antibodies against human lymphocyte antigens].

Verweise: Anti-CD2 Antibody [LT2] (PE) (ab77138)

Abstrakt:

Monoclonal antibodies (McAb) LT1, LT2, LT4, LT6, LT7, LT8, LB21 and LDR to surface antigens of human lymphocytes have been obtained. Basing on the data concerning the distribution of McAb reactivity with cells from different organs and tissues, and with tumour cell lines, on experiments on the mutual blockage and comodulation of antigens, on two-colour immunofluorescence and determination of the molecular weight of the detected antigens a conclusion is made that the McAb obtained react with CD5, CD2, CD4, CD6, CD7, CD8, CD21 and HLA-DR antigens, respectively. McAb LT1 and Leu1, as well as LT2 and Leu5b in pairs blocked each other completely. The determinant recognized by McAb LT8 is more completely overlapped with the epitope, reacting with McAb OKT8. The McAb LT4 binding site is only partially overlapped with the McAb Leu3 site of recognition and practically is not bound to the epitope recognized by McAb OKT4.

Publikation anzeigen