This antibody recognises the rat ED2 cell surface glycoprotein. A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor. Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only. Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.

Anwendungen

Flow Cytometry

Verdünnungen

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Reaktivität

Rat

Immunogen

Rat spleen cell homogenate.

Wirt

Mouse

Klonalität

Monoclonal

Klon

ED2

Isotyp

IgG1

Konjugat

Excitation: 565nm, Emission: 578nm

Reinigung

Protein G affinity chromatography of tissue culture supernatant.

Konzentration

Reconstitution dependent.

Produktform

Lyophilized

Rekonstitution

Reconstitute with 1 ml of distilled water.

Formulierung

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lagerung

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Allgemeine Hinweise

Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007).Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.

Synonyme

C163A_HUMAN, CD 163, CD163 antigen, CD163 molecule, Hemoglobin scavenger receptor, M130, M130 antigen precursor, Macrophage associated antigen, MM130, OTTHUMP00000238617, OTTHUMP00000238618, OTTHUMP00000238619, OTTHUMP00000238620, SCARI1, Scavenger receptor cysteine rich type 1 protein M130, sCD163, Soluble CD163

Isotyp-Kontrollantikörper