Anti-CD163 Antibody [2A10/11] (A279161)

Anti-CD163 Antibody [2A10/11]

Mouse monoclonal [2A10/11] antibody to CD163.

This antibody recognises porcine CD163, a ~120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor. Mouse anti Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner.

WB, IP, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to 1x10⁶ cells in 100µl

Porcine

Porcine alveolar macrophages.

Mouse

Monoclonal

2A10/11

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Approximately 120 kDa in alveolar macrophage cell lysates under non-reducing conditions.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD163 antibody, clone 2A10/11 recognises porcine CD163, a ~120 kDa single pass type 1 transmembrane cell surface glycoprotein expressed on cells of the monocyte/macrophage lineage. The expression levels of CD163 vary during the course of macrophage differentiation. The highest levels of CD163 expression are found on tissue macrophages but bone marrow derived cells are CD163 negative. Expression of CD163 on peripheral blood monocytes varies between about 5% and 50% depending on the donor (Sanchez et al. 1999). Mouse anti Pig CD163, clone 2A10/11 is reported to inhibit both African swine fever infection and viral particle binding to alveolar macrophages in a dose-dependent manner (Sanchez-Torres et al. 2003).

Hemoglobin scavenger receptor, M130, Scavenger receptor cysteine-rich type 1 protein M130