This antibody reacts with CD14, a 53-55 kDa GPI-linked extracellular membrane glycoprotein expressed on monocytes, macrophages, and weakly on granulocytes. This antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.

Anwendungen

Flow Cytometry, IP

Verdünnungen

Flow Cytometry: 2-5 µg/ml.

Reaktivität

Human, Non-Human Primates

Immunogen

A crude mixture of human urinary proteins, precipitated by ammonium sulphate, from the urine of a patient suffering from proteinuria.

Wirt

Mouse

Klonalität

Monoclonal

Klon

MEM-15

Isotyp

IgG1

Konjugat

Biotin

Reinigung

This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.

Konzentration

1 mg/ml

Geschätztes MW

53-55 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Lagerung

Shipped at 4°C. Store at 2-8°C. Do not freeze!

Synonyme

CD 14, CD14 antigen, CD14 molecule, CD14_HUMAN, CD_antigen=CD14, LPS-R, Mo2, Monocyte differentiation antigen CD14, Monocyte differentiation antigen CD14 urinary form, Monocyte differentiation antigen CD14, membrane-bound form, Myeloid cell specific leucine rich glycoprotein, Myeloid cell-specific leucine-rich glycoprotein

Isotyp-Kontrollantikörper

Mouse IgG1 [MOPC-21] (Biotin) (A86204)

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (1)

Bild Vergrößern

Figure 1: Flow Cytometry - Anti-CD14 Antibody [MEM-15] (Biotin) (A85587)

Surface staining of human peripheral blood cells with Anti-CD14 Antibody (A85587).

Produktreferenzen für diesen Klon (1)

Publikation anzeigen

Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.

Verweise: Anti-CD14 Antibody [MEM-15] (Biotin) (ab28118)

Abstrakt:

Monoclonal antibodies against several human leucocyte cell surface antigens were prepared and characterized: B2M-02, a non-cytotoxic antibody against beta-2-microglobulin; MEM-09 and MEM-40, against immunoglobulin kappa-type light chains; MEM-12, MEM-24G and MEM-32B, all against a monomorphic determinant in MHC class II antigens, presumably HLA-DR, dependent on the association of alpha and beta chains; MEM-15 and MEM-18, against a monocyte antigen of 55 kDa; MEM-28, against a 200 kDa antigen expressed on all leucocytes; MEM-31, against the T8 antigen of the cytotoxic/suppressor T-lymphocyte subpopulation; MEM-32, against the T1 antigen of T lymphocytes.

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