This antibody recognises mouse CD137 also known as Tumor necrosis factor receptor superfamily member 9, T-cell antigen 4-1BB or 4-1BB ligand receptor. CD137 is a 256 amino acid ~28 kDa single pass type I transmembrane glycoprotein.CD137 is expressed by activated T lymphocytes, but not by resting cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Mouse
Immunogen
Murine CD137 human Fc fusion protein.
Host
Rat
Clonality
Monoclonal
Clone ID
LOB12
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in TRIS Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Mouse CD137 antibody, clone LOB12 recognizes murine CD137 also known as Tumor necrosis factor receptor superfamily member 9, T-cell antigen 4-1BB or 4-1BB ligand receptor. CD137 is a 256 amino acid ~28 kDa single pass type I transmembrane glycoprotein.CD137 is expressed by activated T lymphocytes, but not by resting cells.