Anti-Cathepsin K Antibody (A84533)

Cathepsin K expression in Rat Skeletal Muscle lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Cathepsin K Antibody (A84533) at 1µg/ml and detected by chemiluminescence.

Cathepsin K expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Cathepsin K Antibody (A84533) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

Cathepsin K expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Cathepsin K Antibody (A84533) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.