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Anti-Cathepsin B Aptamer [Apt08rc] (Biotin) (A321160)

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Vorlaufzeit
Lieferung in 30-45 Werktagen
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+1 (314) 370-6046
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orders@antibodies.com
Name
Anti-Cathepsin B Aptamer [Apt08rc] (Biotin)
Description
Synthetic DNA aptamer to Cathepsin B [Apt08rc] (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: Phosphate Buffered Saline, pH 7.4, with 1mM Calcium Chloride·2 H2O and 0.5mM Magnesium Chloride·6 H2O. Washing Buffer: Binding Buffer, pH 8, Tween 20, and 0.05% (v/v). Binding Temperature: 20°C.
Sequence
5'-dGpdGpdGpdCpdApdGpdGpdCpdApdApdCpdCpdTpdCpdCpdCpdApdCpdTpdApdTpdTpdGpdApdApdGpdApdCpdApdCpdTpdApdCpdCpdApdCpdCpdApdCpdCpdApdCpdTpdGpdCpdApdCpdApdCpdAp-3'
Aptamer Length
50 nt
Aptamer Molecular Weight (Da)
16,500 Da
Aptamer Affinity
Affinity (Kd) = 10.1-10.7 nM
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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